NO-100
MR sensor
Needle sensor
fx-6 UniAmp
Designed for research applications within:
Environmental Sciences
Biomedical Sciences
Biotechnology
Wastewater

NO microsensor

Measure minute concentrations of NO

The NO microsensor offers you a very high sensitivity, and when you add in the fast response time, you get an excellent research tool to measure minute concentrations of NO. Read more...

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Technical Data
Ordering Information & Sizes
Adaptations
Manuals & Videos
NO Microsensor

Measure minute concentrations of NO

Nitric oxide plays an important role in many biological processes. However, natural concentrations are often in the nanomolar range. The Unisense NO microsensor is characterized by a very high sensitivity, which enables you to measure minute concentrations of NO. Combined with a fast response time the Unisense NO microsensor is an excellent research tool for a broad range of tasks.

The sensor can be used equally well for NO measurements in tissue, air, or liquid, and is applied in a broad variety of research fields where non-destructive, fast, and accurate measurements are required.

The NO microsensors are Clark-type sensors measuring the external NO partial pressure. The working principle of the sensor is based on diffusion of NO through a silicone membrane to an NO oxidizing anode, which is polarized against an internal counter cathode. The resulting sensor signal is in the pA range and is measured by a high quality picoammeter, such as the UniAmp Multi Channel.

Compared to other standard NO sensors, the Unisense NO microsensor has an extremely low consumption of max. 935 pmol/hour corresponding to 0.1%/hour in a 1 mL 0.1uM NO sample.

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Characteristics
Stirring Sensitivity & Response Time
Characteristics
Attribute Standard
Lifetime 3 months (min)
Linear range 0-3 µM
Detection limit 15 µm tip 20-30 nM
Detection limit 50 µm tip 4-6 nM
Detection limit 100 µm tip 2-3 nM
Detection limit 500 µm tip 2-3 nM
Zero current (approx.) 15 µm tip 2 pA
Zero current (approx.) 50 µm tip 15 pA
Zero current (approx.) 100 µm tip 25 pA
Zero current (approx.) 500 µm tip 30 pA
Reported Interferents H₂S | NO₂ (gas) | HNO₂ (gas) | Light | TMPD | PMS
Stirring Sensitivity & Response Time
Nitric Oxide sensor Sensitivity (approx.) Stirring Sensitivity (approx.) Response Time (90%) (approx.)
15 µm tip 3 pA/µM 10% <1 s
50 µm tip 15 pA/µM 20% <10 s
100 µm tip 25 pA/µM 25% <10 s
500 µm tip 25 pA/µM 25% <10 s
Ordering information & Sizes
Ordering information & Sizes
Nitric Oxide Sensor Size and description
NO-15 15-20 µm - glass sensor
NO-50 40-60 µm - glass sensor
NO-100 90-110 µm - glass sensor
NO-500 400-600 µm - glass sensor
NO-MR 400-600 µm - in guide
NO-NP 1.6 x 40 mm - needle sensor for piercing
NO-ST-1/4 1/4'' steel tube
NO-SL-1/4 FT-cell 1/4'' Swagelok Tee
NO-SL-1/8 FT-cell 1/8'' Swagelok Tee
NO-PEEK-1/8 FT-cell 1/8'' PEEK Tee
NO-PEEK-1/16 FT-cell 1/16'' PEEK Tee
NO-FT-GLASS-6 Glass FT-cell 6 mm outer diameter
NO-FT-GLASS-8 Glass FT-cell 8 mm outer diameter

Video Guides

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Quick Guide NO Microsensors

Watch this quick guide to learn about the sensor's most important measuring characteristics, specifications, and customizations

Related publications

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Nitric oxide microsensor for high spatial resolution measurements in biofilms and sediments
Schreiber, Frank et all (2008), Analytical Chemistry, vol. 80, 1152-1158
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02
Nitrite-driven anaerobic methane oxidation by oxygenic bacteria
Ettwig, Katharina F. et all (2010), Nature, vol. 464, 543-548
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02
Generation of nitric oxide from nitrite by carbonic anhydrase: a possible link between metabolic activity⁠…
Aamand, Rasmus et all (2009), AJP: Heart and Circulatory Physiology, vol. 297, H2068-H2074
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