NanoRespiration System
Measure extremely low respiration rates
Non-destructive, highly accurate, and reproducible measurements.

NanoRespiration System

Measure respiration of single eggs and embryos, crustaceans, larvae, and more.

With the NanoRespiration System, you can measure respiration rates on single individuals, such as copepod eggs, phyloplankton, or zebrafish eggs. The system has high accuracy and can determine respiration rates down to 0.7 fmol/s. Read more...

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Ordering Information
Manuals & Videos
NanoRespiration System

Measure minute oxygen consumption rates on small organisms

The NanoRespiration System requires exact positioning and moving of the microsensor tip. This is ensured by integration of the NanoRespiration System into the Unisense MicroProfiling System. You can easily operate the MicroProfiling System and log data with SensorTrace Profiling. You can calculate oxygen respiration rates in a spread sheet.

The NanoRespiration System offers

  • Determine extremely low oxygen consumption rates
  • Fast and repetitive measurements of several samples
  • Accurate and non-destructive measurements by Unisense O2 microsensor
  • Autoclavable capillaries
NanoRespiration System rosette
NanoRespiration schematic

NanoRespiration Technology

Samples are placed in oxygen impermeable and chemically inert glass capillaries with sealed bottoms. Each sample is overlaid with appropriate medium. When the setup has reached steady state, an oxygen gradient will build up in the thin capillary. This gradient is measured by a very fine-tipped Unisense microsensor, and the respiration rate of the microorganism placed in the capillary can be calculated from the oxygen gradient.

The NanoRespiration System was developed for determining oxygen respiration rates; however the system is compatible with all Unisense microsensors. In 2006, Riisgaard-Pedersen published a study in Nature in which the NanoRespiration System was used for measuring denitrification by Nonionella cf. stella. based on N2O accumulation in the NanoRespiration Capillaries.

NanoRespiration with sensor
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Ordering information
Ordering information
System components Product
System components
Sensor
Product
OX-50 or other relevant microsensor
System components
Amplifier
Product
UniAmp Multi Channel or another Unisense amplifier
System components
NanoRespirometer
Product
NanoRespirometer (7-capillaries, disc and frame)
System components
Micromanipulator
Product
MM33 or MM33-2 (Single or double head)
System components
Motorized Microprofiling
Product
MOTCON (Motor controller) | MMS (Micromanipulator stage) | MMS-A (Stage adapter)
System components
Software
Product
Software Suite including Profiling Software
System components
Laboratory Stand
Product
LS
System components
Calibration Chamber
Product
CAL300

Applications

Related publications

Respiration rates of individual bovine in vitro-produced embryos measured with a novel, non-invasive and ⁠…
Lopes, Ana S. et al. (2005), Reproduction, vol. 130, 669-679
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Respiration rates of subitaneous eggs from a marine calanoid copepod: Monitored by nanorespirometry
Nielsen, Pernille et al. (2007), Journal of Comparative Physiology B: Biochemical, Systemic, and Environmental Physiology, vol. 177, 287-296
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A coarse-grained nadh redox model enables inference of subcellular metabolic fluxes from fluorescence lifetime imaging
Yang, Xingbo et al. (2021), eLife, vol. 10, -
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