The setup was mounted on an inverted microscope, which allows the user to observe when the sensor tip touches the flask bottom. The sensor is controlled by a motorized micromanipulator and tilted at an angle, allowing the sensor to flex slightly and not break when touching the flask bottom.
The cells are cervical carcinoma cells grown in a 4% O2 atmosphere. During the first 15 days, the cells are passaged twice a week. After seeding on day 15, the medium is changed on day 17 and 19 and the profile and picture shown here is taken on day 20.